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该文探讨了基本培养基、外植体、培养条件以及植物生长调节剂配比对绒毛白蜡(Fraxinus velutina)体胚诱导的影响。结果表明,胚根是诱导体胚发生的最佳外植体;体胚诱导的最适培养基为改良MS+2 mg·L~(–1) 6-BA+0.1 mg·L~(–1) NAA、30g·L~(–1)蔗糖、5.0 g·L~(–1)琼脂;暗培养20天后进行光照培养(14小时光照/10小时黑暗),光密度为100~(–1)20μmol·m~(–2)·s~(–1),昼温度(25±2)°C,夜温度(18±2)°C;成功诱导出体细胞胚并获得再生植株,体胚诱导率可达59.8%,体胚萌发率达81.2%。壮苗最适培养基为改良WPM+0.5 mg·L~(–1) 6-BA+0.2 mg·L~(–1) ZT+0.01 mg·L~(–1) NAA。生根最适培养基为改良1/2MS+1.0 mg·L~(–1)IBA+0.05 mg·L~(–1) NAA+20 g·L~(–1)蔗糖,生根率高达97.3%,试管苗移栽成活率达97.8%。
This article explored the effects of basic media, explants, culture conditions and plant growth regulators on the somatic embryogenesis of Fraxinus velutina. The results showed that the radicle was the best explant for the induction of somatic embryogenesis. The optimum medium for somatic embryo induction was modified MS + 2 mg · L -1 6-BA + 0.1 mg · L -1 ) NAA, 30g · L -1 sucrose and 5.0g · L -1 agar. After dark culture for 20 days, light culture (14h light / 10h dark) 20μmol · m -2 · s -1, day temperature (25 ± 2) ° C and night temperature (18 ± 2) ° C. Somatic embryos were induced successfully and somatic embryos were induced The rate of up to 59.8%, somatic embryo germination rate of 81.2%. The optimum medium for strong seedling was modified WPM + 0.5 mg · L -1 6-BA + 0.2 mg · L -1 ZT + 0.01 mg · L -1 NAA. The optimum medium for rooting was rooting rate as high as 97.3% with 1 / 2MS + 1.0 mg · L -1 IBA + 0.05 mg · L -1 NAA + 20 g · L -1 sucrose, Test tube seedling survival rate of 97.8%.