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目的:建立测定酪酸梭菌肠球菌三联活菌散粒度分布的激光散射法,并与筛分法进行比较。方法:激光散射法测试条件为:振动进样速度为80%,分散气压为0.05 MPa,背景及样品的扫描时间为15 s,遮光度为0.5%~5%,颗粒折射率为1.55,颗粒吸收率为0.01,进样量为0.1~0.2 g。测定粒度分布的特征值粒子体积累计分布图中10%、50%、90%处的粒径值[d(0.1)、d(0.5)、d(0.9)]和体积平均粒径D[4,3]。结果:方法学考察中d(0.1)、d(0.5)、d(0.9)的RSD均小于5%。激光散射法结果显示93.3%的样品粒径小于250μm,64.2%小于150μm,51.4%小于125μm,31.3%小于90μm;筛分法结果显示96.6%的样品粒径小于250μm,46.4%小于150μm,23.5%小于125μm,1.4%小于90μm。结论:筛分法和激光散射法均可以表征样品的粒度分布,但是激光散射法较筛分法的优点是样品量少、重复性好、测量速度快、测量范围广,可以全面表征样品的粒度分布。
Objective: To establish a laser scattering method to determine the particle size distribution of viable Clostridium butyricum enterococci and to compare with the screening method. Methods: The laser scattering method was used for the measurement of the particle size and the particle size. The vibration sampling speed was 80%, the dispersion pressure was 0.05 MPa, the background and sample scanning time was 15 s, the opacity was 0.5% ~ 5% Rate of 0.01, the injection volume of 0.1 ~ 0.2 g. The particle size values (d (0.1), d (0.5), d (0.9)] and the volume average particle size D [4] at 10%, 50% and 90% of the cumulative particle size distribution of the eigenvalues of the particle size distribution were measured. 3]. Results: The RSDs of d (0.1), d (0.5) and d (0.9) in the methodology were less than 5%. The results of laser light scattering showed that 93.3% of the samples were less than 250μm, 64.2% and less than 150μm, 51.4% and less than 125μm, 31.3% and less than 90μm, respectively. The screening results showed that 96.6% of the samples were less than 250μm, 46.4% and less than 150μm, Less than 125 μm, 1.4% less than 90 μm. Conclusion: Both the screening method and the laser scattering method can characterize the particle size distribution of the sample, but the advantage of the laser scattering method is that the sample size is less, the repeatability is good, the measurement speed is fast, the measurement range is wide, and the particle size distributed.