本文利用新生兔的四肢长管骨分离破骨细胞,将其与牛骨片共同培养。24小时后,培养液中加入白细胞介素2(IL-2),使终浓度分别为50μ/ml和100μ/ml,并做空白对照。培养40小时、64小时及1周,采用相差显微镜计数每个骨片上形成的吸收陷窝数,同时拍照,再利用图象分析系统计算每张照片上吸收陷窝的表面积。初步的研究结果表明:IL-2可以刺激破骨细胞性骨吸收。 In this paper, osteoclasts were isolated from the long bones of newborn rabbits and co-cultured with bovine bone slices. After 24 hours, interleukin 2 (IL-2) was added to the culture to a final concentration of 50 μL / ml and 100 μL / ml, respectively, and a blank control was made. After culturing for 40 hours, 64 hours and 1 week, the number of lacunae formed on each bone piece was counted by a phase-contrast microscope, and pictures were taken at the same time. Then, the image analysis system was used to calculate the surface area of ​​the lacunae on each picture. Preliminary results show that: IL-2 can stimulate osteoclastic bone resorption.