本文旨在建立一种适用于大豆子叶的RNA和DNA同步抽提方法,得到高质量的核酸以满足后续实验要求。该方法先用异硫氰酸胍裂解大豆子叶,再用常规苯酚/氯仿抽提去除杂质,最后用LiCl选择性沉淀核酸。核酸的完整性和纯度检测表明抽提的总RNA和DNA质量良好,进一步的RT-PCR和PCR检测显示抽提的总RNA和DNA质量适合后续的实验要求。 This article aims to establish a synchronous extraction of RNA and DNA suitable for soybean cotyledons to obtain high quality nucleic acids to meet the requirements of subsequent experiments. The method uses guanidine isothiocyanate to cleave soybean cotyledons, then uses conventional phenol / chloroform extraction to remove impurities, and finally uses LiCl to selectively precipitate the nucleic acid. The integrity and purity of the nucleic acids showed that the extracted total RNA and DNA were of good quality. Further RT-PCR and PCR tests showed that the extracted total RNA and DNA quality were suitable for the subsequent experiments.