论文部分内容阅读
目的:建立高效液相色谱法测定脂降宁片中葛根素、二苯乙烯苷的含量。方法:采用kromasil C18柱(4.6 mm×250 mm,5μm);流动相为甲醇(A)-水(B),梯度洗脱;流速1.0 mL/min;检测波长为250nm及320nm;柱温:30℃。结果:葛根素进样量在232～29μg范围内(r=0.9998),二苯乙烯苷进样量在220～19.5μg范围内(r=0.9997)线性关系良好,平均回收率葛根素为97.2%(RSD=0.8%),二苯乙烯苷为97.6%(RSD=0.7%)。结论:该方法操作简便,分离度高,重复性好,可同时测定脂降宁片中葛根素、二苯乙烯苷的含量。
Objective: To establish a method for the determination of puerarin and stilbene glycosides in Zhijinning Tablets by high performance liquid chromatography. Methods: Kromasil C18 column (4.6 mm × 250 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of methanol (A) -water (B) with gradient elution. The flow rate was 1.0 mL / min. The detection wavelength was 250 nm and 320 nm. ℃. Results: The injection volume of puerarin was in the range of 232 ~ 29μg (r = 0.9998), and the linear range was 220 ~ 19.5μg (r = 0.9997). The average recoveries of puerarin were 97.2% (RSD = 0.8%) and stilbene glucoside was 97.6% (RSD = 0.7%). Conclusion: The method is simple and easy to operate with high resolution and good reproducibility. The method can be used for the simultaneous determination of puerarin and stilbene glycosides in Zhijinning tablets.