诺氟沙星软膏为喹诺酮类抗菌药,收载于《中国药典》1995年版二部,其规定的含量测定方法为经过萃取后用紫外分光光度法测定,该法提取费时,结果误差较大。本文建立了反相ＨＰＬＣ法测定诺氟沙星软膏含量,方法简便,重现性好,专属性强。1　仪器与试剂岛津ＬＣ-5Ａ高效液相色谱仪,岛 Norfloxacin ointment quinolone antibacterial drugs, contained in the “Chinese Pharmacopoeia” 1995 edition two, the provisions of the determination of the content after extraction by UV spectrophotometry, the method of extracting time-consuming, the results of larger errors. This article established a reversed-phase HPLC method for the determination of norfloxacin ointment content, the method is simple, reproducible, highly specific. 1 instruments and reagents Shimadzu LC-5A high performance liquid chromatography, the island