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利用红松种子的单倍体胚乳,采用水平切片淀粉凝胶电泳技术,对采自三个地区的天然红松林的材料进行了10种酶系统的等位酶实验,共获得了18个等位基因位点,对这18个位点等位基因的统计分析表明:红松种群的多态位点百分数为P=77.78%,等位基因平均数为A=2.0,预期杂合度为He=0.1648。红松种群间遗传分化程度在松属植物中属于较低水平(Gst=5.38%),在红松18个等位基因位点的变异中,绝大部分变异94.62%来自种群内部。根据Nei方法计算的红松三个种群的遗传一致度和遗传距离为地理较近的小北湖与长白山地区种群的遗传距离最小(0.0095),而地理距离最远的长白山种群与凉水种群的遗传距离(0.0143)却小于凉水种群与小北湖种群间的遗传距离(0.0166),表明了这三个种群在起源上的复杂性。
Using the haploid endosperm of Korean pine seeds, 10 isozyme system experiments were conducted on the materials of natural Korean pine forests collected from three regions using horizontal slice starch gel electrophoresis. A total of 18 alleles The statistical analysis of the 18 loci alleles showed that the percentage of polymorphic loci in P. koraiensis population was 77.78%, the average number of alleles was A = 2.0, and the predicted heterozygosity He = 0.1648. The genetic differentiation of Pinus koraiensis populations was lower in Pinus (Gst = 5.38%). Among the 18 alleles of Pinus koraiensis, most of the variation was 94.62% . The genetic identity and genetic distance of the three populations of Pinus koraiensis based on the Nei method are the least (0.0095). The geographical distance between the Changbai Mountain and the Changbai Mountain is the smallest The genetic distance (0.0143) was less than the genetic distance (0.0166) between cold water and Xiaobeihu populations, indicating the origin of the three populations complexity.