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用１０~（－３）ｍｏｌ／Ｌ叠氮化钠（ＮａＮ_３）作为诱变剂处理大麦种子，收取Ｍ_２世代籽粒用香草醛－盐酸试剂进行半粒分析，含原花色素者剖面出现红色反应，缺失者无反应，以此筛选获得一系列原花色素缺失的突变体。对Ｍ_３和Ｍ_４世代的分析结果表明，突变体的原花色素缺失性状是稳定的，但大多数突变体的抗逆性较弱，早衰较严重，使籽粒灌浆受到影响，粒重和饱满度下降，需要进一步改良。但也有个别突变体如甘ａｎｔ９０－５等具有较好的综合性状，接近对照品种。
Barley seeds were treated with 10 ~ (-3) mol / L sodium azide (NaN_3) as mutagen, and seeds of M_2 generation were collected for semi - particle analysis with vanillin - hydrochloric acid reagent. Lack of those who did not respond, in order to screen a series of pro-anthocyanidin mutants. The results of M_3 and M_4 generations showed that the mutant was stable in proanthocyanidin, but most of the mutants had weaker resistance and premature aging, which affected the grain filling, decreased grain weight and fullness , Need to be further improved. But there are some mutants such as Gan ant90-5 have better comprehensive traits, close to the control varieties.