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目的:明确虫草菌丝对四氯化碳小鼠肝纤维化的作用及肝脏脂质过氧化的影响。方法:四氯化碳皮下注射复制BALB/c小鼠肝纤维化模型,虫草组在造模同时予以虫草菌丝煎剂,直至造模结束。HE染色观察肝组织炎症,天狼猩红染色观察肝脏胶原沉积,生化法测定肝组织羟脯氨酸(Hyp)、超氧化物歧化酶(SOD)含量,Nothern Blot测定I型前胶原mRNA。结果:与正常小鼠比较,肝纤维化小鼠肝脏肝静脉与汇管区周围肝细胞明显脂肪变性,肝脏胶原沉积,可见纤维间隔形成,肝脏Hyp含量及I型前胶原基因表达均显著增加,肝脏S0D减少;与模型组比较,虫草组肝脏炎症与胶原沉积减轻(P<0.01),Hyp含量及I型前胶原mRNA表达明显降低(P<0.01),SOD活性明显增加(P<0.01)。结论:虫草菌丝制剂有良好的抗肝纤维化作用,其作用机制可能提高肝脏SOD水平、降低I型前胶原mRNA的表达有关。
Objective: To clarify the effect of Cordyceps sinensis mycelium on carbon tetrachloride-induced liver fibrosis and liver lipid peroxidation. Methods: The model of hepatic fibrosis in BALB / c mice was subcutaneously injected with carbon tetrachloride. The Chinese caterpillar fungus group was treated with Cordyceps sinensis at the same time as modeling, and the model was finished. Hepatic inflammation was observed by HE staining. Liver collagen deposition was observed by Sirius red staining. Hyp and superoxide dismutase (SOD) contents were measured by biochemical method. Precollagen type I mRNA was detected by Nothern Blot. Results: Compared with normal mice, hepatocellular hepatic veins in hepatic fibrosis mice and hepatic cells around the portal area were significantly steatosis, hepatic collagen deposition, fibrous septa formation, liver Hyp content and type I procollagen gene expression were significantly increased, the liver (P <0.01). Compared with the model group, the hepatic inflammation and collagen deposition of Cordyceps decreased (P <0.01), the content of Hyp and the expression of procollagen I (P <0.01) and the activity of SOD increased significantly (P <0.01). CONCLUSION: Cordyceps mycelium has a good anti-hepatic fibrosis effect, and its mechanism of action may improve the level of liver SOD and decrease the expression of type I procollagen mRNA.