1961年海尔弗列克(Hayflick)博士首先报告了人胚肺二倍体成纤维细胞在体外培养时具有有限的传代次数。实验是在培养瓶中进行的,从人胚肺分离出成纤维细胞培养在瓶中,当细胞长满瓶底以后,等分传代,再长满,再分种,如此一次次传种,约50次时细胞开始萎缩,衰老和死亡。正常的二倍体细胞在体外分种传代的次数有一个极限值,人们将此值称为海尔弗克极限。人体 In 1961, Dr. Hayflick first reported that human embryonic lung diploid fibroblasts have a limited number of passages when cultured in vitro. Experiments are carried out in the flask, isolated from human embryonic lung fibroblasts cultured in bottles, after the cells covered the bottom of the bottle, the equal sub-passage, and then full, and then sub-species, so time and time, around 50 times the cells began to shrink, aging and death. There is a limit to the number of normal diploid cells subcultured in vitro, which is called the Haier Fuke limit. human body