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目的比较研究不同类型抗瘤中药制剂对结直肠癌细胞分泌免疫抑制分子的影响。方法分别制备三氧化二砷(As2O3)、川芎嗪(LHC)、黄芪(AMB)、氧化苦参碱(MOX)、猪苓多糖(PUPS)、青蒿琥酯(ART)等6种中药制剂作用后的小鼠结直肠癌细胞Colon26再培养上清;以不经中药制剂作用的相应上清为对照。定量酶联免疫吸附法(ELISA)测定上清中TGF-β1、VEGF、IL-4、IL-6、IL-10和PGE2含量。结果在6种中药制剂对Colon26分泌免疫抑制分子的影响中,AMB对TGF-β1的下调作用最强(55.75%);As2O3、LHC对IL-6的下调幅度均约5%,但作用时相不同;As2O3对IL-10的下调作用最强(7.35%);对VEGF、IL-4及PGE2的影响之间差异无统计学意义(均P>0.05)。结论 TGF-β1可能是结直肠癌细胞分泌的优势免疫抑制分子,是各种中药制剂下调免疫抑制的重要靶分子;下调肿瘤细胞免疫抑制分子的分泌,应是中药制剂发挥抗瘤效应的机制之一。
Objective To compare the effects of different types of anti-tumor traditional Chinese medicine preparations on the secretion of immunosuppressive molecules in colorectal cancer cells. Methods Six kinds of traditional Chinese medicine preparations such as arsenic trioxide (As2O3), ligustrazine (LHC), astragalus (AMB), oxymatrine (MOX), polyporus polysaccharide (PUPS) and artesunate (ART) were prepared. The colorectal cancer cell Colon26 was used to re-incubate the supernatant; the corresponding supernatant without the effect of the traditional Chinese medicine preparation was used as a control. Quantitative enzyme-linked immunosorbent assay (ELISA) was used to determine the contents of TGF-β1, VEGF, IL-4, IL-6, IL-10 and PGE2 in the supernatant. Results In the effect of six traditional Chinese medicine preparations on the immunosuppressive molecules secreted by Colon26, AMB had the strongest down-regulation effect on TGF-β1 (55.75%); As2O3 and LHC down-regulated the extent of IL-6 by about 5%, but the effect phase Different; As2O3 had the strongest down-regulation of IL-10 (7.35%); there was no significant difference in the effect of VEGF, IL-4 and PGE2 (all P>0.05). Conclusions TGF-β1 may be the dominant immunosuppressor molecule secreted by colorectal cancer cells and it is an important target molecule for downregulation of immunosuppression by various traditional Chinese medicine preparations; down-regulation of the immunosuppressor molecule secretion of tumor cells should be the mechanism by which TCM preparations exert an anti-tumor effect. one.