用聚丙烯酰胺凝胶等电点聚焦电泳法,比较研究了微小按蚊(广义)卵、二龄和四龄幼虫、蛹及雌性成虫蛋白质和酯酶同工酶电泳图谱。微小按蚊(广义)不同发育期蛋白质等电点pH值,卵、幼虫、蛹和成虫显带区在pH4.1～9.5,其中在酸性区显带最多,碱性次之;酯酶同工酶等电点pH值显带区在pH4.2～7.8,其中酸性区显带最多,中性次之。并对微小按蚊复合体作了讨论。 Polyacrylamide gel electrophoresis was used to study the protein and esterase isoenzymes of Anopheles minimus (broad sense) eggs, second and fourth instar larvae, pupae and female adults. The isoelectric point (pH) of egg protein, egg, larva, pupa and adult of Anopheles minimus (broad sense) at different developmental stages ranged from pH4.1 to 9.5, of which the bands were the most in the acidic zone and followed by the alkaline; Enzyme isoelectric point pH value banding zone at pH4.2 ~ 7.8, of which acidic zone banding most, followed by neutral. Anopheles minimus complex was also discussed.