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本文研究了分别用谷氨酸脱羧酶和大肠杆菌作为催化L-谷氨酸脱羧的酶材料来制作的生物传感器的响应性能。结果表明这两种电极各具特点。谷氨酸脱羧酶电极对底物L-谷氨酸的校正曲线的线性范围为3.16×10~(-4)mol/L～1.0×10~(-2)mol/L。斜率为46mV,响应时间为6～8min电极寿命仅为5天。而大肠杆菌膜电极线性范围为4.22×10~(-4)mol/L～1.78×10~(-2)mol/L,斜率为48.5mV,响应时间为8～9min,电极寿命达34天。这两种电极对底物L-谷氨酸皆具有专一的选择性,且在磷酸吡哆醛的存在下,活性都有所增加。将电极用于味精中L-谷氨酸钠的测定,获得较满意的结果。
In this paper, we studied the response of biosensors prepared with glutamic acid decarboxylase and Escherichia coli respectively as enzyme materials that catalyze the decarboxylation of L-glutamic acid. The results show that the two electrodes have their own characteristics. The calibration curve of glutamic acid decarboxylase electrode to substrate L-glutamic acid showed a linear range of 3.16 × 10 -4 mol / L -1.0 × 10 -2 mol / L. The slope of 46mV, the response time of 6 ~ 8min electrode life is only 5 days. The linear range of E.coli membrane electrode was 4.22 × 10 -4 mol / L ~ 1.78 × 10 -2 mol / L, the slope was 48.5 mV, the response time was 8 ~ 9 min and the electrode life was 34 days. Both of these electrodes have a specific selectivity for the substrate L-glutamic acid and have increased activity in the presence of pyridoxal phosphate. The electrode was used in the determination of sodium monosodium glutamate, to obtain more satisfactory results.