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作者报道,美国一直在强调检查污水和国内废水处理工厂处理后的流出物中有无沙门氏菌。为此人们正努力寻求简易快速的方法对这种致病菌进行分离、鉴别、计数。近来,Brooker 等人研究出了一种非常简单迅速的试验法来检查肠杆菌属的赖氨酸脱羧酶活性(简称 LD 试验)。用此法1～4小时内便可得出结果。由于这种试验法简易、迅速且具有特异性,作者已用作早期确定沙门氏菌。将水样用力摇动使之充分混匀并将1ml 接种到盛有10ml 考夫曼四硫酸盐肉汤的试管中,以10ml 或者更多量接种到盛有同等容量浓缩两倍的培养基的试管或烧瓶中。把全部已接种
The authors report that the United States has been emphasizing the inspection of effluents and the presence or absence of Salmonella in treated effluents from domestic wastewater treatment plants. To this end people are trying to find a quick and easy way to isolate, identify and count this pathogen. Recently, Brooker et al. Have developed a very simple and rapid test for the detection of Lysine decarboxylase activity in Enterobacter (referred to as LD test). Use this method within 1 to 4 hours to get the result. Because of the ease, speed and specificity of this assay, the authors have used Salmonella for early identification. The water sample was vigorously shaken to mix well and 1 ml was inoculated into a test tube containing 10 ml of Kauffman tetrathionate broth and inoculated into a test tube containing 10% or more of the same volume of concentrated medium Or in the flask. Have all been vaccinated