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目的观察中成药血必净注射液对体外培养的小鼠脑微血管内皮细胞株(bEnd.3)增殖活力的影响,探讨其促进内皮损伤修复的药物效应。方法采用小鼠脑微血管内皮细胞株bEnd.3,分别给予质量浓度为0、5、25和50 mg/ml血必净刺激12、24和48 h后,应用四甲基偶氮唑蓝法(MTT)检测各组细胞的增殖情况,并应用流式细胞仪检测血必净对细胞周期(增殖指数%=(G7-M)%+S%)的影响。采用单因素方差分析或非参数秩和检验进行统计学分析。结果与对照组(0 mg/ml)相比,血必净5 mg组和25 mg组在12 h和24 h的MTT和增殖指数均明显升高(P<0.05),两组在48 h的增殖指数明显降低(P<0.05),而MTT则差异无统计学意义;50 mg组的MTT在12 h与对照组相比差异无统计学意义(P>0.05),而在24 h和48 h则显著降低(P<0.05);其增殖指数在12 h和24 h均明显升高(P<0.01),在48 h显著下降(P<0.01)。结论血必净对于体外培养的小鼠脑微血管内皮细胞在特定药物浓度和时间内具有刺激增殖的作用,提示对内皮细胞的损伤具有一定的修复作用。
Objective To observe the effect of Xuebijing injection of Chinese patent medicine on the proliferation of mouse brain microvascular endothelial cell line (bEnd.3) cultured in vitro, and to explore the drug effect of promoting the repair of endothelial injury. METHODS: Mouse brain microvascular endothelial cell line bEnd.3 was used and given a concentration of 0, 5, 25, and 50 mg/ml of Xuebijing for 12, 24, and 48 h, respectively, followed by tetramethyl azoline blue method ( MTT assay was used to detect the proliferation of cells in each group, and the effect of Xuebijing on the cell cycle (proliferation index%=(G7-M)%+S%) was detected by flow cytometry. Statistical analysis was performed using one-way analysis of variance or non-parametric rank sum test. Results Compared with the control group (0 mg/ml), the MTT and proliferation index at 12 h and 24 h in the Xuebijing 5 mg group and the 25 mg group were significantly increased (P<0.05). The two groups were at 48 h. The proliferation index was significantly lower (P<0.05), but there was no statistically significant difference in MTT. There was no significant difference in the MTT of the 50 mg group compared with the control group at 12 h (P>0.05), but at 24 h and 48 h. Significantly decreased (P<0.05); its proliferation index was significantly increased at 12 h and 24 h (P<0.01), and decreased significantly at 48 h (P<0.01). Conclusion Xuebijing has the function of stimulating proliferation in mouse brain microvascular endothelial cells cultured in vitro at specific drug concentration and time, suggesting that it has certain repairing effect on endothelial cell injury.