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目的 研究氟对人胚肝细胞 (L 0 2细胞 )氧化应激、DNA损伤及诱导凋亡的作用。方法 体外培养的L 0 2细胞接触 40、 80、 160 μg/ml氟化钠 2 4h ,检测L 0 2细胞脂质过氧化物 (LPO)水平 ,还原型谷胱甘肽 (GSH )含量 ,DNA损伤率 ,细胞凋亡率和周期构成比的情况。结果 氟可使L 0 2细胞LPO水平升高 ,GSH含量下降 ,并且二者均与氟浓度呈明显的剂量 效应关系 ;氟可使L 0 2细胞DNA损伤率、凋亡率和S期细胞数明显升高 ,高剂量氟组DNA损伤率和凋亡率与低剂量氟组之间存在显著性差异。结论 氟可致L 0 2细胞出现氧化应激 ,引起DNA损伤 ,诱导细胞凋亡 ,并且三者之间存在明显的相关关系
Objective To investigate the effects of fluoride on oxidative stress, DNA damage and apoptosis in human hepatocytes (L 0 2 cells). Methods L 0 2 cells in vitro were exposed to 40, 80, 160 μg / ml sodium fluoride for 24 h. The levels of LPO, GSH, Injury rate, rate of apoptosis and cycle composition ratio of the situation. Results Fluorocarbon increased LPO level and GSH level in L 0 2 cells, and both of them showed a dose-dependent effect on fluoride concentration. Fluorocarbon induced DNA damage rate, apoptosis rate and number of S phase cells in L 0 2 cells Significantly increased, high-dose fluorine group DNA damage rate and apoptosis rate and low-dose fluorine group there was a significant difference. Conclusion Fluorine can cause oxidative stress in L 0 2 cells, induce DNA damage and induce apoptosis, and there is a clear correlation between the three