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miRNA(microRNA)是一类21～24个核酸长度的非编码小分子RNA(sRNA),它主要通过抑制或降解靶基因来调控植物生长发育等过程。试验利用在纤维长度上有显著差异的2个回交自交系BILs(Backcrossinbred lines)的0 DPA(Days post anthesis)、3 DPA的胚珠和10 DPA的纤维构建6个sRNA文库并进行Solexa测序。以已公布的棉花D5基因组序列和棉属其他序列为参考,经分析共发现561个miRNAs,其中包括254个已知的miRNAs(属于40个miRNA家族),75个候选的miRNAs和232个新的miRNAs,研究结果极大地丰富了棉属miRNAs。通过miRNA靶基因预测分析发现多数miRNAs负调控其对应的靶基因,少数正调控。KEGG(Kyoto encyclopedia of genes and genomes)注释结果表明miRNA的靶基因在植物激素代谢途径中显著富集。
MicroRNAs (miRNAs) are a class of non-coding small RNAs (sRNAs) with a length of 21-24 nucleotides. They regulate the growth and development of plants mainly by inhibiting or degrading target genes. Six sRNA libraries were constructed and Solexa sequenced using 0 DPA (Days post anthesis) of 2 backcross inbred lines (BILs), 3 DPA ovules and 10 DPA fibers with significant differences in fiber length. A total of 561 miRNAs were found, including 254 known miRNAs (belonging to 40 miRNA families), 75 candidate miRNAs and 232 new ones, based on the published cotton D5 genomic sequences and other cotton sequences. miRNAs, the results greatly enriched cotton miRNAs. By miRNA target gene prediction analysis found that most miRNAs negatively regulate its corresponding target gene, a small number of positive regulation. The results of the KEGG (Kyoto encyclopedia of genes and genomes) annotation indicate that miRNA target genes are significantly enriched in plant hormone metabolic pathways.