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目的研究两种抗应激剂对恒河猴热应激的影响。方法将6只恒河猴随机分为A,B两组采血做9项血液学及9项血液生化指标测定,A组日粮中每只添加VC300 mg/d和VE50 mg/d;B组日粮中添加0.2%苜草素,饲喂两个月后分别将A、B两组恒河猴放入(32±1)℃,湿度70%猴房内,1 h后采血,测定相应血液学及血液生化指标。结果两组动物血液学各项指标试验前后差异不显著(P>0.05);而血液生化值呈现:A组试验后AST、ALP、LDH分别为(101±23.64)U/L,(623.67±233.12)U/L,(1384.33±807.66)U/L,试验前后差异不显著(P>0.05);B组试验后TP、ALB分别为(83.83±16.32)g/L,(38.53±0.57)g/L;试验前后差异不显著。结论VC和VE在恒河猴抗热应激过程中主要作用于维持血液中AST、ALP、LDH的活性稳定,而苜草素主要作用于维持血液中TP、ALB的稳定,两种添加剂能有效提高恒河猴抗热应激的能力。
Objective To study the effects of two anti-stress agents on heat stress in rhesus monkeys. Methods 6 rhesus monkeys were randomly divided into A and B blood sampling to do 9 hematology and 9 blood biochemical indexes. Each group A diet was supplemented with VC300 mg / d and VE50 mg / d. Group B 0.2% alfalfa was added to the diet, and after two months of feeding, Rhesus monkeys in groups A and B were respectively placed in (32 ± 1) ℃ and 70% And blood biochemical indicators. Results There was no significant difference in hematological parameters between the two groups before and after the experiment (P> 0.05). The blood biochemical values showed that the levels of AST, ALP and LDH in group A were (101 ± 23.64) U / L, (623.67 ± 233.12 (P <0.05). The TP and ALB in group B were (83.83 ± 16.32) g / L and (38.53 ± 0.57) g / L, L; the difference was not significant before and after the experiment. Conclusions VC and VE play a major role in maintaining the activity of AST, ALP and LDH in the blood of Rhesus macaques in the course of anti-heat stress, while the control of TP and ALB in the blood is effective for the stability of TP and ALB in blood Improve the ability of rhesus monkey to resist heat stress.