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目的探讨T-cadherin分子表达抑制胶质母细胞瘤C6细胞增殖的分子机制。方法利用脂质体将pcDNA3和pcDNA3.T-cadherin表达质粒转染C6细胞,筛选出表达T-cadherin的C6克隆3、4和5以及转染空载体后不表达T-cadherin的C6克隆1和2,以Western Bolt检测各克隆的p21和p27的表达;同时,以pcDNA3和pcDNA3.T-cadherin表达质粒分别转染野生型(p21+/+)和p21缺失突变型(p21-/-)成纤维细胞,研究T-cadherin分子介导的细胞增殖抑制是否依赖细胞周期蛋白p21的表达。结果转染pcDNA3.T-cadherin质粒后表达T-cadherin分子的C6克隆3、4和5的p21表达水平明显升高,而p27的表达与C6细胞是否表达T-cadherin无关。另外,转染pcDNA3.T-cadherin质粒的野生型表达p21的成纤维细胞的增殖显著受抑,而转染pcDNA3.T-cadherin质粒对p21缺失突变的成纤维细胞的增殖不产生抑制作用。结论T-cadherin分子抑制胶质母细胞瘤C6细胞的增殖与p21表达密切相关,且T-cadherin分子对成纤维细胞的增殖抑制作用依赖于p21的表达。
Objective To investigate the molecular mechanism of T-cadherin expression inhibiting the proliferation of glioblastoma C6 cells. Methods C6 cells were transfected with pcDNA3 and pcDNA3.T-cadherin plasmids by lipofectamine. Sixty-four clones 3, 4 and 5 expressing T-cadherin and C6 clone 1 without expressing T-cadherin The expression of p21 and p27 in each clone was detected by Western Bolt. At the same time, the wild type (p21 + / +) and p21 deletion mutant (p21 - / -) fibroblasts were transfected with pcDNA3 and pcDNA3.T-cadherin expression plasmids respectively Cells to investigate whether T-cadherin-mediated inhibition of cell proliferation depends on the expression of cyclin p21. Results The expression of p21 in C6, 3, 4 and 5 cells transfected with pcDNA3.T-cadherin plasmid was significantly increased, while the expression of p27 was not associated with the expression of T-cadherin in C6 cells. In addition, the proliferation of wild type p21-expressing fibroblasts transfected with pcDNA3.T-cadherin plasmid was significantly inhibited, while the transfection of pcDNA3.T-cadherin plasmid did not inhibit the proliferation of p21-deficient fibroblasts. Conclusion T-cadherin inhibits the proliferation of C6 cells in glioblastoma is closely related to the expression of p21, and the inhibitory effect of T-cadherin on fibroblast proliferation depends on the expression of p21.