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目的:建立HPLC法测定仙鹿胶囊中淫羊藿苷的含量。方法:采用YMC C18分析柱(150 mm×4．6 mm,5μm),流动相:甲醇-水(55:45),检测波长:270 nm,流速:1．0 ml·min-1,柱温:25℃。结果:淫羊藿苷在0．1-1．0μg范围内与峰面积呈良好的线性关系,r=0．999 9(n=5),平均回收率为98．6％,RSD为1．3％。结论:本法准确可靠,简便灵敏,重复性好,可用于仙鹿胶囊的质量控制。
Objective: To establish HPLC method for the determination of icariin in Xianlu capsule. Methods: A YMC C18 column (150 mm×4.6 mm, 5 μm) was used. The mobile phase was methanol-water (55:45). The detection wavelength was 270 nm. The flow rate was 1.0 ml·min-1. : 25°C. RESULTS: There was a good linear relationship between icariin and peak area in the range of 0.1-1.0 μg, r=0.999 9 (n=5), the average recovery was 98.6%, RSD was 1. 3%. Conclusion: This method is accurate and reliable, simple and sensitive, good repeatability, can be used for the quality control of Xianlu capsule.