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目的探讨EB病毒(EBV)抗体及血清EBV—DNA载量检测在诊断儿童EB病毒感染传染性单核细胞增多症中的意义。方法收集2013年2月~2014年9月间入住我院住院治疗的120例EBV感染传染性单核细胞增多症患儿,采用间接免疫荧光法检测EBV特异性抗体及抗体亲和力,荧光定量PCR方法检测血清中EBV-DNA核酸。结果在120例儿童EBV感染传染性单核细胞增多症病例中,入院时血清EBV抗体反应存在多种类型,抗EBV-CA-Ig M阳性率达93.4%。120例进行血清EBV-DNA检测,总阳性率为38.3%(45、120)。结论血清EBCA-Ig M结合EBV-CA-Ig G亲和力检测,可以提高EBV感染传染性单核细胞增多症诊断的敏感度。血清中EBV-DNA检测阳性率较低,故不推荐对IM患者进行常规EBV-DNA检测,但对病程长,症状反复,病情重的患儿应监测血浆中EBV-DNA,有助于评估治疗的效果及预后。EBV-CA-Ig M阴性病人可提高诊断IM的灵敏度。
Objective To explore the significance of detecting Epstein-Barr virus (EBV) antibody and serum EBV-DNA load in the diagnosis of Epstein-Barr virus infection in children with infectious mononucleosis. Methods A total of 120 EBV-infected infectious mononucleosis patients admitted to our hospital from February 2013 to September 2014 were collected. The antibody-specific antibody and antibody affinity of EBV were detected by indirect immunofluorescence. Fluorescent quantitative PCR Detection of serum EBV-DNA nucleic acid. Results Among the 120 EBV-infected infectious mononucleosis patients, there were many types of serum EBV antibody responses at admission and the positive rate of anti-EBV-CA-Ig M was 93.4%. 120 cases of serum EBV-DNA test, the total positive rate was 38.3% (45,120). Conclusion The serum EBCA-Ig M combined with EBV-CA-Ig G affinity test can improve the sensitivity of EBV infection in the diagnosis of infectious mononucleosis. However, EBV-DNA testing is not recommended in patients with IM, but EBV-DNA in plasma should be monitored in children with long course of symptoms, repeated symptoms and severe illness, which is helpful to evaluate the treatment The effect and prognosis. EBV-CA-Ig M-negative patients can improve the sensitivity of the diagnosis of IM.