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制约大豆生产的因素很多,土壤盐渍化就是其中之一。大量研究证明过表达NHX逆向转运蛋白可以提高植物的耐盐性。为获得耐盐性良好的转基因大豆材料,我们将大豆Na~+/H~+逆向转运蛋白(GmNHX1)基因构建到植物表达载体pCAMBIA3300上,应用农杆菌介导法将GmNHX1导入大豆品种黑农56和黑农59中,共获得18个转基因株系,并对T_1代转基因株系进行了PCR和实时荧光定量PCR检测。PCR结果表明转基因后代株系呈阳性的植株有4株;经Real-time PCR检测该4个PCR阳性转基因株系的G NHX1基因表达水平均高于对照株系;200 mmol/L NaCl溶液的盐试结果表明;对照植株的生长速度滞缓于4个转基因株系的生长速度。
There are many factors that restrict soybean production. Soil salinization is one of them. Numerous studies have shown that overexpression of NHX antiporter enhances plant salt tolerance. In order to obtain transgenic soybeans with good salt tolerance, we constructed the GmNHX1 soybean Na ~ + / H ~ + antiporter (GmNHX1) gene into plant expression vector pCAMBIA3300. Agrobacterium-mediated transformation of GmNHX1 into soybean cultivar Heinong 56 And Heinong59, 18 transgenic lines were obtained, and the T_1 transgenic lines were detected by PCR and real-time fluorescence quantitative PCR. The results of PCR showed that there were 4 plants positive for transgenic progenies. The expression of G NHX1 gene in 4 PCR-positive transgenic lines was higher than that of control by Real-time PCR. The salt of 200 mmol / L NaCl solution The test results showed that the growth rate of the control plants was slowed to the growth rate of the four transgenic lines.