论文部分内容阅读
目的探索一名出生时乙肝疫苗免疫成功后仍感染乙肝病毒的小孩的感染来源。方法采集小孩及其父母的血标本进行HBV血清学标志物和病毒载量检测,使用酚氯仿法提取HBV DNA,PCR扩增HBV S基因,将扩增产物进行克隆并测序,利用Mega 5.0和Bioedit 7.0对测序结果进行分析。结果父亲和母亲均为隐匿性乙型肝炎病毒感染,父亲、母亲、儿子的PCR产物分别获得12、11和9个克隆株。父亲克隆株的血清型为adrq+,ayw1,ayw,ayr,基因型为C2、B、B/C;母亲克隆株的血清型为ayw1,adw2,adwq+,基因型为C5、B、B/C、C/G;儿子克隆株的血清型为adrq+,基因型仅为C2。S基因序列进化发生树显示,儿子的所有克隆序列与父亲大多数的克隆序列聚集在一起,而与母亲的克隆序列没有聚集。来自儿子和父亲的S基因克隆序列有相同的氨基酸突变模式(T118K、T123N、G145A)。结论儿子乙型肝炎病毒感染的来源是父亲,提示隐匿性乙型肝炎病毒感染可通过家庭密切接触传播。
Objective To explore the source of infection in a child who is still infected with hepatitis B virus after the successful immunization of hepatitis B vaccine at birth. Methods Blood samples of children and their parents were collected for HBV serological markers and viral load detection. The HBV DNA was extracted by phenol-chloroform method, and the HBV S gene was amplified by PCR. The amplified products were cloned and sequenced. Mega 5.0 and Bioedit 7.0 Analysis of sequencing results. Results Both father and mother were occult hepatitis B virus infection, and 12, 11 and 9 clones were obtained respectively from the PCR products of father, mother and son. The serotypes of the cloned parent strains were adrq +, ayw1, ayw and ayr, and the genotypes were C2, B and B / C. The serotypes of the maternal clone were ayw1, adw2 and adwq +, and the genotypes were C5, B and B / C / G; son clone strains serotype adrq +, genotype only C2. The phylogenetic tree of S gene sequences showed that all of the cloned sequences of the sons were clustered with most of the father’s cloned sequences and did not aggregate with the mother’s cloned sequences. The S gene clone sequences from sons and daughters have the same pattern of amino acid mutations (T118K, T123N, G145A). Conclusion The source of hepatitis B virus infection in his son is the father, suggesting that occult hepatitis B virus infection can be transmitted by close family contact.