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目的观察桃红四物汤对肾脏缺血再灌注损伤(renal ischemic reperfusion injure,RIRI)大鼠的保护作用,并探讨其作用机制。方法 Wistar大鼠50只,随机分为A组(假手术组)、B组(缺血再灌注模型组)、C组(硝苯地平阳性对照组)、D组(桃红四物汤预处理低剂量组)、E组(桃红四物汤预处理高剂量组),各10只。A组、B组大鼠以生理盐水灌胃,C组大鼠以硝苯地平0.5 g/kg灌胃,D组、E组大鼠以桃红四物汤(生药浓度1 g/ml)5、10 g/kg灌胃。连续给药14 d,1次/d。第14天给药后麻醉大鼠,经腹正中切口暴露双侧肾脏,游离双侧肾蒂,右肾结扎。A组不夹闭左侧肾蒂,暴露60 min后缝合腹腔切口。其他各组用无创动脉夹夹闭左侧肾动脉,60 min后松开使血流再灌注,缝合腹腔。再灌注24 h后下腔静脉采血,检测血尿素氮(blood urea nitrogen,BUN)、肌酸酐(creatinine,Cr)、丙二醛(malondialdehyde,MDA)含量和超氧化物歧化酶(superoxide dismutase,SOD)活性,光镜下观察左肾形态学变化。计量资料采用单因素方差分析,两两比较采用LSD-t检验,P<0.05为差异有统计学意义。结果 B组大鼠血清中Cr、BUN、MDA含量[(456.20±53.78)μmol/L、(17.32±0.86)mmol/L、(8.38±0.56)nmol/L]较A组的(151.78±25.75)μmol/L、(9.13±0.95)mmol/L、(5.70±0.62)nmol/L明显升高;B组SOD活性为(417.50±23.48)NU/ml,较A组的(458.90±32.59)NU/ml降低,差异均有统计学意义(均P<0.05)。D组和E组的血清Cr、BUN、MDA含量分别为(298.45±30.41)μmol/L、(12.93±0.74)mmol/L、(5.98±0.68)nmol/L、(272.87±33.45)μmol/L、(12.24±1.23)mmol/L、(5.72±0.56)nmol/L,较B组均显著降低,D组和E组SOD活性[(533.65±42.54)、(546.50±33.82)NU/ml]较B组明显升高,差异均有统计学意义(均P<0.05)。结论桃红四物汤可改善缺血再灌注大鼠肾脏功能,其机制可能与抗氧自由基作用有关。
Objective To observe the protective effect of Taohong Siwu Tang on renal ischemia / reperfusion injure (RIRI) rats and to explore its mechanism. Methods Fifty Wistar rats were randomly divided into three groups: sham operation group, sham operation group, ischemia / reperfusion group, C group (nifedipine positive control group), D group Dose group), E group (Taohong Siwu Decoction high-dose group), each 10. Rats in group A and group B were given gavage with physiological saline. Rats in group C were treated with nifedipine 0.5 g / kg, while rats in groups D and E were given Taohong Siwu Decoction (crude drug concentration 1 g / ml) 10 g / kg gavage. Continuous administration of 14 d, 1 / d. On the 14th day, the rats were anesthetized, the bilateral kidney was exposed through the median incision, the free bilateral renal pedicle and the right kidney were ligated. Group A did not clamp the left renal pedicle, sutured abdominal incision 60 minutes after exposure. In the other groups, the left renal artery was occluded with a noninvasive artery clip, and the blood stream was reperfused after 60 min, and the abdominal cavity was sutured. Blood was collected from the inferior vena cava 24 h after reperfusion, and the levels of blood urea nitrogen (BUN), creatinine (Cr), malondialdehyde (MDA) and superoxide dismutase (SOD) ) Activity, morphological changes observed under light microscope. Measurement data using one-way analysis of variance, pairwise comparison using LSD-t test, P <0.05 for the difference was statistically significant. Results The contents of Cr, BUN and MDA in group B were significantly higher than those in group A (151.78 ± 25.75) (456.20 ± 53.78 μmol / L, 17.32 ± 0.86 mmol / L, 8.38 ± 0.56 nmol / L, (417.50 ± 23.48) NU / ml in group B was significantly higher than that in group A (458.90 ± 32.59) NU / ml, the differences were statistically significant (all P <0.05). The levels of serum Cr, BUN and MDA in group D and E were (298.45 ± 30.41) μmol / L, (12.93 ± 0.74) mmol / L, (5.98 ± 0.68) nmol / L and (272.87 ± 33.45) μmol / L , (12.24 ± 1.23) mmol / L and (5.72 ± 0.56) nmol / L, respectively, which were significantly lower than those in group B. SOD activities in groups D and E were 533.65 ± 42.54 and 546.50 ± 33.82 NU / ml, respectively B group was significantly higher, the difference was statistically significant (P <0.05). Conclusion Taohongsiwutang can improve renal function in rats with ischemia-reperfusion, the mechanism may be related to the role of antioxidant free radicals.