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为了研究内含子在活体水平对凝血因子Ⅸ的表达增强作用，首先采用lacZ为报告基因，建立了阳离子脂质体SA介导的活体基因转移方法。将带有凝血因子Ⅸ（hFⅨ）cDNA的质粒pCMVIX和带有hFⅨcDNA及其内含子的小基因（minigene）的质粒pCMVi’Ⅸ用SA脂质体包裹，分别经尾静脉注射到Balb／c鼠体内，pCMVIX注射后未在血浆中检测到hFⅨ蛋白；而pCMVi’Ⅸ注射后在Balb／c鼠血浆中检测到hFⅨ蛋白，最高达到10．1ng／ml，持续28天。pCMVi’Ⅸ经二次注射，FⅨ表达水平最高达到43.9ng／ml，持续40天后仍可检测到hFⅨ基因的表达，结果表明，内含子在活体水平对凝血因子Ⅸ的表达有显著的增强作用。
In order to study the role of intron in enhancing the expression of coagulation factor Ⅸ in vivo, we first used lacZ as the reporter gene to establish a cationic liposome-mediated live gene transfer method. The plasmid pCMVIX with the factor IX (hFIX) cDNA and the plasmid pCMVi’IX with the hFIX cDNA and its intron were introduced into SA liposomes and injected into the tail vein of Balb / c mice In vivo, hFⅨ protein was not detected in plasma following injection of pCMVIX; hFⅨ protein was detected in plasma of Balb / c mice after injection of pCMVi’Ⅸ up to 10.1 ng / ml for 28 days. The expression level of hFIX gene in pCMVi’Ⅸ was up to 43.9ng / ml after secondary injection, and the expression of hFⅨ gene was still detected after 40 days. The results showed that intron had a significant enhancement on the expression of coagulation factor Ⅸ .