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目的检测类风湿关节炎(RA)患者血清肿瘤坏死因子受体相关因子1(TRAF1)水平,并分析其与RA疾病活动性关系,探讨其与CD40在RA发病机制中的共同作用。方法采集2015年7月至2015年12月苏州大学附属第一医院门诊及住院部70例初诊RA患者和50名健康体检者外周血,记录RA患者临床及实验室指标,酶联免疫吸附法(ELISA)检测TRAF1、CD40,并分析两者相关性。结果 RA患者TRAF1、CD40分别为(35.88±51.22)ng/L,(72.70±70.56)ng/L,与健康对照组[TRAF1和CD40分别为(12.51±8.59)ng/L和(31.54±27.11)ng/L]相比差异均有统计学意义(P<0.05)。根据DAS28评分,将RA患者分为高、中、低活动期组,3组TRAF1分别为(13.05±4.29)ng/L、(29.84±42.48)ng/L和(58.84±66.90)ng/L,差异有统计学意义(F=6.382,P=0.003),且TRAF1与DAS28评分呈正相关(r=0.419,P<0.001)。RA患者血清TRAF1与自身抗体RF、GPI呈正相关(r=0.365,P=0.001;r=0.767,P<0.001)。RA患者血清TRAF1表达与CD40呈正相关(r=0.465,P<0.001)。结论 TRAF1在RA患者明显增高,与RA疾病活动性、自身抗体和CD40呈正相关。
Objective To detect the serum level of tumor necrosis factor receptor related factor 1 (TRAF1) in patients with rheumatoid arthritis (RA) and analyze its relationship with the activity of RA and explore its synergism with the pathogenesis of RA in patients with RA. Methods Seventy patients with newly diagnosed RA patients and 50 healthy volunteers from the First Affiliated Hospital of Soochow University from July 2015 to December 2015 were enrolled in this study. The clinical and laboratory parameters of RA patients were recorded. Enzyme linked immunosorbent assay ELISA) TRAF1, CD40, and analyze the correlation between the two. Results The levels of TRAF1 and CD40 in RA patients were (35.88 ± 51.22) ng / L and (72.70 ± 70.56) ng / L, respectively, which were significantly higher than those in healthy controls (12.51 ± 8.59 ng / L and 31.54 ± 27.11, ng / L] compared with the difference was statistically significant (P <0.05). According to the DAS28 score, RA patients were divided into high, medium and low activity groups. The TRAF1 levels were (13.05 ± 4.29) ng / L, (29.84 ± 42.48) ng / L and (58.84 ± 66.90) ng / L, The difference was statistically significant (F = 6.382, P = 0.003). There was a positive correlation between TRAF1 and DAS28 scores (r = 0.419, P <0.001). RA patients serum TRAF1 and autoantibodies RF, GPI was positively correlated (r = 0.365, P = 0.001; r = 0.767, P <0.001). TRAF1 expression in RA patients was positively correlated with CD40 (r = 0.465, P <0.001). Conclusion TRAF1 is significantly increased in patients with RA and positively correlated with the activity of RA, autoantibodies and CD40.