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目的:调查大理吸毒人群乙型肝炎病毒(HBV)的基因型和亚型。方法:采集大理市某戒毒所吸毒人群血清共192份,采用酶联吸附免疫法(ELISA)测定HBV血清标志物(HBVM),再采用基因型特异性引物PCR和聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定HBV感染血清基因型和亚型。结果:192份血清中,HBsAg阳性率8.85%(17/192)。17份HBsAg阳性标本中基因型分别为:B型3例,均为Ba型;C型1例,为Cs型;B+C混合型3例(Ba+Cs);未扩增10例。结论:大理吸毒人群HBV感染率较高;HBV感染的基因型为B型、C型和B+C型,基因亚型为Ba和Cs。
Objective: To investigate the genotypes and subtypes of hepatitis B virus (HBV) in Dali drug users. Methods: A total of 192 serum samples from 192 drug addicts in Dali City were collected for detection of serum HBV markers (HBVMs) by enzyme-linked immunosorbent assay (ELISA). Genotype-specific PCR and polymerase chain reaction-restriction fragments PCR-RFLP was used to determine the genotypes and subtypes of HBV infection. Results: Among 192 serum samples, the positive rate of HBsAg was 8.85% (17/192). The genotypes of 17 HBsAg positive specimens were: type B in 3 cases, all in type Ba; type C in 1, type Cs; type B + C in 3 (Ba + Cs); Conclusion: The HBV infection rate in Dali drug addicts is high. The genotypes of HBV infection are B type, C type and B + C type, and the genotypes are Ba and Cs.