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目的 构建 p5 3重组腺病毒载体以研究 p5 3过度表达与腺病毒感染对细胞生长的影响 .方法 将 p5 3c DNA克隆到转移载体 p Ad CMV/ p5 3重组质粒 ,用该重组质粒及p JM1 7质粒共转染 2 93细胞获得重组腺病毒 ,PCR法筛选含p5 3c DNA的重组病毒 .结果 在挑选的 5个空斑中 ,有 4个含 p5 3c DNA阳性重组腺病毒 .p5 3重组腺病毒可感染 2 93细胞并在 2 93细胞内进行有效的复制 .结论 成功构建了 p5 3重组腺病毒 ,为进一步研究 p5 3重组腺病毒的功能提供了条件
Objective To construct p5 3 recombinant adenovirus vector to study the effect of p5 3 overexpression and adenovirus infection on cell growth.Methods p5 3c DNA was cloned into pAd CMV / p5 3 recombinant plasmid and transfected with p JM13 Plasmids were co-transfected into 293 cells to obtain recombinant adenoviruses, and PCR was used to screen recombinant viruses containing p5 3c DNA.Results Of the 5 plaques selected, 4 contained recombinant adenovirus containing p5 3c DNA.p5 3 recombinant adenovirus Which could infect 293 cells and effectively replicate in 293 cells.Conclusion The successful construction of p5 3 recombinant adenovirus provided the basis for further study on the function of p5 3 recombinant adenovirus