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[目的]探讨肠复康方对急性放射性肠炎大鼠一般情况及小肠黏膜相关炎性因子的影响。[方法]以X线直线加速器给予实验大鼠全腹照射(DT9.0Gy),建立急性放射性肠炎大鼠模型。实验大鼠随机分成正常对照组(NC)、模型对照组(MC)、肠复康方低剂量治疗组(CFKD-LG)、肠复康方中剂量治疗组(CFKD-MG)、肠复康方高剂量治疗组(CFKD-HG)。造模成功后连续30d灌胃,期间观察对比大鼠精神状态、摄食进水、排便情况及体质量变化,处死后取相应部位的小肠用RT-PCR方法检测黏膜上皮细胞中炎性因子IL-1β、IL-6、TNF-αmRNA的表达。[结果]造模成功后肠复康方低、中、高剂量组精神状态、饮食进水、排便情况较模型对照组好;与模型对照组比较,肠复康方低、中、高剂量组大鼠体质量显著增加,肠复康方中剂量组效优(P<0.01)。肠复康方低、中、高剂量组小肠黏膜的炎性因子IL-1β、IL-6、TNF-αmRNA较模型对照组显著下调(P<0.01)。[结论]肠复康方低、中、高剂量组通过下调小肠黏膜上皮细胞中炎性因子IL-1β、IL-6、TNF-αmRNA的表达,改善小肠黏膜的炎症反应,促进急性放射性肠炎受损小肠黏膜的修复,以中剂量效优。
[Objective] To investigate the effects of Changfukang Recipe on the general situation and the inflammatory factors of intestinal mucosa in rats with acute radiation enteritis. [Method] The whole rat abdominal irradiation (DT9.0Gy) was given by X-ray linear accelerator to establish the rat model of acute radiation enteritis. The experimental rats were randomly divided into normal control group (NC), model control group (MC), Changfukangfang low dose treatment group (CFKD-LG), Changfukangfang middle dose treatment group (CFKD-MG) Fang high-dose treatment group (CFKD-HG). The model rats were given intragastric administration for 30 days continuously. Mental status, water intake, defecation and body weight of the rats were observed and compared. RT-PCR was used to detect the expression of inflammatory cytokines IL- 1β, IL-6, TNF-αmRNA expression. [Result] Compared with the model control group, the state of mental health, water intake and defecation of food in the low, middle and high dosage groups of Changfukang, The body weight of rats increased significantly, and the middle dose of Changfukang Recipe was effective (P <0.01). The intestinal mucosal IL-1β, IL-6 and TNF-αmRNA of Changfukang Recipe in low, medium and high dose groups were significantly lower than those in model control group (P <0.01). [Conclusion] Changfukangfang low, medium and high dose group can reduce the inflammatory reaction of small intestine mucosa by reducing the expression of inflammatory cytokines IL-1β, IL-6 and TNF-αmRNA in intestinal mucosal epithelial cells and promote acute radiation enteritis Loss of small intestinal mucosa repair, effective in medium dose.