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目的 获得DYS4 37,A7 1,H4三个Y染色体STR基因座及其单倍型在北京汉族人群中的遗传多态性分布 ,并探讨其法医学应用价值。方法 应用自行建立的Y STR 15 plex复合扩增体系 ,对用酚 /氯仿法提取的 132份北京地区汉族无关男性个体血样DNA样品进行复合扩增 ,用ABI310型遗传分析仪对扩增产物进行检测 ,统计分析 3个Y STR基因座的群体遗传学参数。结果 DYS4 37,A7 1,H4三个Y STR基因座在该群体中分别检出 4 ,5 ,4个等位基因 ,GD值分别为 0 4 977,0 6 731,0 5 42 0 ;观察到 32种单倍型 ,其中 17种单倍型出现 1次 ,最多 1种单倍型出现 2 0次 ,单倍型累积GD值为 0 9118。结论 3个Y STR基因座具有较强的个体识别能力 ,在法医学个体识别和亲权鉴定中具有很高的应用价值
Objective To obtain the distribution of genetic polymorphisms of DYS4 37, A7 1, H4 three Y chromosome STR loci and their haplotypes in Beijing Han population and to explore the value of forensic medicine. METHODS: The Y STR 15 plex multiplex amplification system was used to amplify the DNA samples from 132 blood samples of unrelated Han individuals in Beijing area. The amplified products were detected by ABI310 genetic analyzer The population genetic parameters of three Y STR loci were statistically analyzed. Results Three Y STR loci of DYS4 37, A7 1 and H4 were detected in the population of 4, 5, 4 alleles, GD values were 0 4 977,0 6 731,0 5 42 0; observed 32 haplotypes, of which 17 haplotypes appeared once, up to 1 haplotype appeared 20 times, haplotype cumulative GD value was 0 9118. Conclusion The three Y STR loci have strong ability of individual identification and have high value in forensic identification and paternity testing