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A fuzzless-lintless (fl) seed mutant of Gossypium hirsutum L. cv. Xu-142 was investigated to study cotton fiber development. Scanning electron microscopy revealed that fiber initials were virtually absent from fl ovules. RT-PCR analysis showed that the steady-state levels of transcripts of the fiber-specific E6 and Expansin genes were high in wild type (WT) ovules during the fiber initiation and elongation stages, and peaked around 15 days post anthesis (DPA), but only a trace amount of these transcripts was detectable in fl ovules of alt developmental stages investigated. CotmybA, a member of the Myb family, exhibited a clear expression in developing WT ovules, but the expression was abnormal in fl ovules. Application of GA3, or GA3 plus IAA, to the culture medium rescued in vitro fiber initiation and growth of fl ovules only partially. In addition, transcription of E6 and Expansin genes of in vitro cultured WT and fl ovules responded similarly to exogenous hormones. The hormones had less effect on Cot
A fuzzless-lintless (fl) seed mutant of Gossypium hirsutum L. cv. Xu-142 was investigated to study cotton fiber development. Scanning electron microscopy revealed that fiber initials were virtually absent from fl ovules. RT-PCR analysis showed that the steady- state levels of transcripts of the fiber-specific E6 and Expansin genes were high in wild type (WT) ovules during the fiber initiation and elongation stages, and peaked around 15 days post anthesis (DPA), but only a trace amount of these transcripts was detectable in fl ovules of alt developmental stages investigated. CotmybA, a member of the Myb family, exhibited a clear expression in developing WT ovules, but the expression was abnormal in fl ovules. Application of GA3, or GA3 plus IAA, to the culture medium rescued in vitro fiber initiation and growth of fl ovules only partially. In addition, transcription of E6 and Expansin genes of in vitro cultured WT and fl ovs directly to analogous exogenous hormones. The hormones had less effect on Cot