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目的探讨绒毛细胞长期培养法在产前诊断及早孕和早中孕期自然流产病因检测中的应用价值及两者有无差异。方法采用绒毛细胞长期培养法分别对147例B超引导下经腹绒毛取材的产前诊断病例及384例自然流产患者的流产绒毛进行染色体培养制作、核型分析以及结果对照比较。结果产前诊断绒毛培养成功率为96.60%,明显高于自然流产绒毛的培养成功率(87.24%);两者的平均培养天数无明显差异(11.5天vs12.3天);流产绒毛染色体异常率(63.88%)明显高于产前诊断绒毛(9.15%)。结论绒毛细胞长期培养法是一种有效的检测绒毛染色体的方法 ,同时适用于产前诊断绒毛和流产绒毛;绒毛标本的无菌性、新鲜程度对绒毛细胞培养成功率有着重要影响。
Objective To investigate the value of long-term culture of villus cells in prenatal diagnosis and early pregnancy and early pregnancy in the etiology of spontaneous abortion and its significance. Methods Chorionic culture of hair cells from 147 prenatal diagnosed cases of B-ultrasound-guided abortion and 384 spontaneous abortions were performed by long-term culture of villus cells. Karyotype analysis and results were compared. Results The success rate of prenatal diagnosis of villus culture was 96.60%, which was significantly higher than that of spontaneous abortion (87.24%). There was no significant difference between the two groups (11.5 days vs. 12.3 days) (63.88%) was significantly higher than prenatal diagnosis of villi (9.15%). Conclusion Long-term culture of villus cells is an effective method for the detection of chorion chromosomes. It is also suitable for prenatal diagnosis of villi and abortion villus. The asexuality and freshness of villus specimens have an important influence on the success rate of villus cell culture.