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p16基因又名多肿瘤抑制基因(MTS1),1994年首先由Kamb et al克隆成功。此基因包含3个外显子及2个内含子,位于人9号染色体短臂2区1带(9p21),编码P16蛋白,在人类多种肿瘤中存在纯合性缺失及突变等变异,但胃癌中的改变情况国内外报道较少。p18基因是一个新近发现的与p15,p16基因同属一个基因家庭的抑癌基因,在结构和功能上与p15,p16基因具有高度同源性,但在多种人类癌肿中其变异少见,它在胃癌中的改变情况目前国内外尚未见报道。本研究通过PCR及银染PCR-SSCP技术检测胃癌组织p16基因外显子E1a,E1β,E2及p18基因外显子E1的纯合性缺失及突变情况。 1 材料和方法 1.1材料 43例胃癌患者的新鲜癌手术标本、相应的癌旁正常组织置-70℃冰箱中保存备用。本组病例中男27例,女16
The p16 gene, also known as the multi-tumor suppressor gene (MTS1), was first cloned by Kamb et al in 1994. This gene contains three exons and two introns, located in the short arm of human chromosome 9 in region 1 (9p21), encoding P16 protein in human tumors exist homozygous deletions and mutations and other mutations, However, the changes in gastric cancer less reported at home and abroad. The p18 gene is a recently discovered tumor suppressor gene in the same gene family as the p15 and p16 genes. It has a high degree of homology to the p15 and p16 genes in structure and function, but its mutation is rare in many human cancers The changes in gastric cancer have not been reported at home and abroad. In this study, the homozygous deletion and mutation of exon E1a, E1β, E2 and exon E1 of p16 gene in gastric cancer tissue were detected by PCR and silver staining PCR-SSCP. 1 Materials and Methods 1.1 Materials 43 cases of gastric cancer patients with fresh cancer specimens, the corresponding normal tissue next to -70 ℃ refrigerator spare. In this group of patients, 27 males and 16 females