510.6nm激光对脐血造血细胞的增殖作用

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介绍了510.6nm激光对造血细胞的刺激作用,促使颗粒-巨噬细胞集落形成单位(GM-CFUc)增多。从人脐带血中分离制备的有核细胞中,加入集落刺激因子(CSF)后进行体外培养,形成的GM-CFUc为18.6±12.6/104;如果加入的CSF是经激光照射后的细胞制成的,其GM-CFUc为40.5±20.2/104;如果先用激光照射脐带血的有核细胞,在培养时加入的CSF是未经激光照射后的细胞制成的,也有增殖效应,GM-CFUc为36.5±18.5/104;如果脐带血中的有核细胞和制备CSF的细胞都用激光照射,发现GM-CFUc明显增多,其值为57.4±41.2/104,是未经激光处理者的三倍多 The stimulating effect of 510.6nm laser on hematopoietic cells was introduced, leading to the increase of granulocyte-macrophage colony forming unit (GM-CFUc). GM-CFUc was 18.6 ± 12.6 / 104 when cultured in vitro and cultured in human umbilical cord blood after addition of colony stimulating factor (CSF). If the added CSF was irradiated by laser Of cells with a GM-CFUc of 40.5 ± 20.2 / 104; if umbilical cord blood cells were first irradiated with laser, the CSF added during the culture was made of cells that had not been irradiated with laser , And also had a proliferative effect with GM-CFUc of 36.5 ± 18.5 / 104. If both umbilical cord blood cells and CSF-producing cells were irradiated with laser, a significant increase of GM-CFUc was found with a value of 57.4 ± 41.2 / 104, more than three times of those without laser treatment
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