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目的 探讨人类白细胞抗原 (HLA) DRB1和 DQB1等位基因多态性是对乙型肝炎病毒 (HBV)易感性及干扰素 (IFN)抗HBV治疗的影响。方法 应用聚合酶链反应 序列特异性引物 (PCR SSP)技术检测上海地区 6 9例慢性乙型肝炎 (CHB)患者的HLA DRB1和 DQB1等位基因以及 2 0 0名健康准骨髓捐献者的HLA DRB1等位基因。其中 32例接受α 1b干扰素治疗 2 4周。结果 CHB患者HLA DRB1 0 6和 DRB1 0 8等位基因的频度高于健康人 (2 .17%对 0 % ,RR =3.96 3,95 %CI :3.4 2 5～ 4 .5 85 ,P =0 .0 17;11.5 9%对 5 .5 0 % ,RR=2 .2 5 3,95 %CI:1.14 7～ 4 .4 2 8,P =0 .0 2 1) ;而DRB1 0 7等位基因的频度则低于健康人 (2 .90 %对 7.75 % ,RR=0 .35 5 ,95 %CI:0 .12 3～ 1.0 2 5 ,P =0 .0 4 7)。IFN治疗的患者中 ,10例应答者DRB1 14等位基因频度高于 2 2例非应答者 (2 0 .0 %对 2 .3% ,RR =10 .75 0 ,95 %CI :1.116～ 10 3.5 5 8,P =0 .0 30 ) ;而DQB1 0 7分布则相反(10 .0 %对 38.6 % ,RR =0 .176 ,95 %CI :0 .0 36～ 0 .85 6 ,P =0 .0 2 2 )。结论 HLA等位基因多态性可能与上海地区人群HBV的易感性和IFN抗HBV治疗有关。与其他HLA DRB1等位基因比较 ,HLA DRB1 0 6和 DRB1 0 8可能与HBV易感性有关 ,而HLA DRB1 0 7则相反 ,?
Objective To investigate the effects of human leukocyte antigen (HLA) DRB1 and DQB1 alleles on susceptibility to hepatitis B (HBV) and anti-HBV treatment of interferon (IFN). Methods The HLA DRB1 and DQB1 alleles in 69 chronic hepatitis B (CHB) patients and the HLA DRB1 in healthy donors were detected by polymerase chain reaction sequence specific primers (PCR SSP) Alleles. 32 of them received α 1b interferon for 24 weeks. Results The frequencies of HLA DRB1 0 6 and DRB1 0 8 alleles in CHB patients were significantly higher than those in healthy subjects (2.17% vs 0%, RR = 3.96 3, 95% CI: 3.425-5.55, P = 0.17; 11.5 9% versus 5.5%, RR = 2.523, 95% CI: 1.14 7-4.482, P = 0.021); while DRB107 etc. The frequency of the bit genes was lower than that in healthy individuals (2.90% vs. 7.75%, RR = 0.3535, 95% CI: 0.123 to 1.025, P = .047). The frequency of DRB1 14 alleles in 10 responders was higher than that in 22 non-responders (20.0% vs. 2.3%, RR = 10.75 0, 95% CI: 1.116 ~ 10 3.5 5 8, P = 0 .0 30); while the distribution of DQB1 0 7 was opposite (10 .0% vs 38.6%, RR = 0.176, 95% CI: 0.36 ~ 0.856, P = 0 .0 2 2). Conclusion HLA allele polymorphism may be related to HBV susceptibility and IFN anti-HBV therapy in Shanghai population. Compared with other HLA DRB1 alleles, HLA DRB1 0 6 and DRB1 0 8 may be related to HBV susceptibility, while HLA DRB1 0 0 is the opposite.