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目的:研究多西紫杉醇对人胃癌中分化细胞株SGC7901的作用效果及机制。方法:MTT法检测多西紫杉醇对胃癌细胞株SGC7901的增殖抑制作用。AnnexinV方法检测药物作用后胃癌细胞的凋亡。PI染色法检测凋亡晚期的胃癌细胞。采用流式细胞仪检测多西紫杉醇作用前后细胞凋亡相关分子Fas蛋白、Bcl2蛋白表达水平的变化。结果:0.92、3.7、14.8、59.2μg/mL多西紫杉醇作用于SGC7901细胞72h,抑制率分别为13.3%、21.6%、57.5%、61.0%。多西紫杉醇可诱导胃癌细胞株SGC7901发生细胞凋亡。多西紫杉醇使SGC7901凋亡分子Fas表达增加,作用前后分别为(26.5±7.2)%、(37.9±7.0)%;多西紫杉醇作用SGC7901前后Bcl2表达分别为(38.9±9.1)%、(31.2±5.6)%,差异无显著性。结论:多西紫杉醇对胃癌中分化细胞株SGC7901生长有明显的抑制作用,可诱导胃癌细胞系SGC7901凋亡,凋亡作用的发生可能与多西紫杉醇诱导Fas分子表达有关。
Objective: To study the effect and mechanism of docetaxel on human gastric cancer cell line SGC7901. Methods: MTT assay was used to detect the inhibitory effect of docetaxel on the proliferation of gastric cancer cell line SGC7901. AnnexinV method was used to detect apoptosis of gastric cancer cells after drug treatment. PI staining was used to detect gastric cancer cells in late stage of apoptosis. Flow cytometry was used to detect the changes of apoptosis-related molecules Fas and Bcl2 protein expression before and after docetaxel treatment. Results: After treated with docetaxel 0.92, 3.7, 14.8 and 59.2μg / mL for 72h, the inhibitory rates were 13.3%, 21.6%, 57.5% and 61.0%, respectively. Docetaxel can induce apoptosis of gastric cancer cell line SGC7901. Docetaxel increased the expression of Fas in SGC7901 apoptotic cells (26.5 ± 7.2)% and (37.9 ± 7.0)%, respectively. Before and after treatment with docetaxel, the expression of Bcl2 was (38.9 ± 9.1)% and (31.2 ± 5.6)%, no significant difference. CONCLUSION: Docetaxel can significantly inhibit the growth of SGC7901 gastric cancer cell line and induce the apoptosis of gastric cancer cell line SGC7901. The apoptosis may be related to the induction of Fas molecule by docetaxel.