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目的:观察臭常山根(Orixae japonicae)对家兔离体肠肌自发活动的影响,并探讨其作用机制。方法:以家兔离体肠肌收缩强度为指标[即以肠肌舒缩活动的张力(g)、收缩幅度及收缩频率为指标],观察不同浓度的臭常山根水提物对家兔离体肠平滑肌收缩的影响。结果:不同浓度的臭常山根水提物(终质量浓度按生药量计为0.25,0.5,1 g.mL-1)均能显著抑制家兔正常离体十二指肠的自发活动,但不是完全抑制肠肌的自发活动,能使家兔正常离体肠肌舒缩活动的张力减小,收缩力减弱,紧张性下降;对乙酰胆碱和BaCl2引起的肠肌强直收缩均有显著拮抗作用,并且能使先用阿托品紧张性降低的离体家兔肠肌进一步松弛,且随剂量增加抑制作用增强。结论:臭常山根水提物能显著抑制家兔离体十二指肠的自发活动,使收缩力减弱,紧张性下降,能拮抗乙酰胆碱和BaCl2引起的肠肌痉挛,并能协同阿托品抑制肠肌的收缩,呈量效关系,其抑制效应可能通过胆碱能受体介导或对肠平滑肌直接起作用。
OBJECTIVE: To observe the effect of Orixae japonicae on the spontaneous activity of isolated rat intestinal muscle and to explore its mechanism of action. Methods: The strength of intestine muscles in isolated rabbits was used as an index[ie, the tension (g), contraction amplitude, and contraction frequency of intestinal muscle relaxation and contraction activities] were used as indicators to observe the effects of different concentrations of odorant root water extract on rabbits. Intestinal smooth muscle contraction effects. RESULTS: The concentration of odor extract of Changshan root with different concentrations (0.25, 0.5, 1 g.mL-1 in terms of crude drug concentration) could significantly inhibit the spontaneous activity of isolated duodenum in rabbits, but not Completely inhibiting the spontaneous activity of the intestinal muscles can reduce the tension of the normal isolated muscles of the intestine, reduce the contractility, and reduce the tension; it has significant antagonistic effects on acetylcholine and BaCl2-induced contraction of intestinal muscles. It can further relax the isolated rabbit intestinal muscle with atropine tension first, and the inhibitory effect increases with increasing dose. Conclusion: The root extract of Odorantia philoxeroides can significantly inhibit the spontaneous activity of the isolated duodenum in rabbits, weaken the contractility, and reduce the tension. It can antagonize the intestinal muscle spasm caused by acetylcholine and BaCl2, and can inhibit intestinal muscle in collaboration with atropine. The contraction is in a dose-effect relationship, and its inhibitory effect may be mediated by cholinergic receptors or act directly on intestinal smooth muscle.