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目的探讨miR-487a调节米托蒽醌(MX)耐药的乳腺癌耐药细胞MCF-7/MX对MX敏感性的作用与机制。方法通过在乳腺癌耐药细胞MCF-7/MX中转染miR-487amimic构建miR-487a高表达乳腺癌耐药细胞模型,应用(qRT)-PCR、蛋白印迹法、流式等方法检测miR-487a通过靶向作用乳腺癌耐药蛋白BCRP增加乳腺癌细胞对MX的敏感性。结果在MCF-7/MX细胞中miR-487a高表达抑制BCRP的表达,增加MX的细胞内蓄积,提高该细胞对MX的敏感性。结论 miR-487a通过靶向作用BCRP,增加乳腺癌细胞对MX的敏感性。
Objective To investigate the effect and mechanism of miR-487a on MX sensitivity of mitoxantrone-resistant breast cancer cell line MCF-7 / MX. Methods miR-487amimic was transfected into multidrug resistant breast cancer cell line MCF-7 / MX to construct a miR-487a-overexpressing breast cancer cell line. The expression of miR-487a was detected by qRT- 487a increases the sensitivity of breast cancer cells to MX by targeting the breast cancer resistance protein BCRP. Results High expression of miR-487a in MCF-7 / MX cells inhibited the expression of BCRP, increased the intracellular accumulation of MX and increased the sensitivity of the cells to MX. Conclusion miR-487a can increase the sensitivity of breast cancer cells to MX by targeting BCRP.