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目的:将介导金黄色葡萄球菌侵袭细胞的关键蛋白FnBP的重要功能肽段与GST蛋白在大肠杆菌中融合表达.方法:利用合成的特异性引物钓取金黄色葡萄球菌基因组中的目的片段,将其克隆入pGEX-KG载体,在大肠杆菌中与GST蛋白进行融合表达.结果:成功地表达了目的肽,可溶性的表达产物占细菌可溶性蛋白的20%左右,且目的肽与配基纤连蛋白Fn具有结合的能力.结论:成功地表达了介导金黄色葡萄球菌侵袭细胞的关键蛋白FnBP中的重要功能肽段,为研究金葡菌侵袭细胞的机制奠定了基础.
OBJECTIVE: To fuse and express the important functional peptide of FnBP, a key protein involved in the invasion of Staphylococcus aureus, with GST protein in Escherichia coli.METHODS: The target fragment of Staphylococcus aureus genome was ligated by using specific primers, The recombinant plasmid was cloned into pGEX-KG vector and fused with GST protein in Escherichia coli.Results: The target peptide was expressed successfully and its soluble expression product accounted for about 20% of the bacterial soluble protein, and the target peptide and the fibronectin Protein Fn had the binding ability.Conclusion: The successful expression of key functional FnBP-mediated peptides that affect the invasion of S. aureus cells lay the foundation for studying the mechanism of S. aureus invasion of cells.