论文部分内容阅读
目的探讨食品添加剂明矾对大鼠脑组织中三磷酸腺苷(ATP)酶活性以及钙、镁元素含量的影响。方法选择雄性Wistar大鼠32只,按体重随机分为4组,对照组、低、中、高剂量明矾组,连续喂养30 d后测定大鼠脑组织中Na+K+-ATP酶、Ca2+-ATP酶、Mg2+-ATP酶活性及脑组织中铝、钙、镁元素含量,并进行脑组织病理切片观察。结果低、中、高剂量明矾组脑组织中铝含量分别为(2.19±0.39)、(2.48±0.33)、(3.52±0.41)μg/g,明显高于对照组(0.52±0.12)μg/g(P<0.01);高剂量组大鼠脑组织中镁元素含量、Mg2+-ATP酶活性明显低于对照组(P<0.05);低、中、高剂量组Na+K+-ATP酶活性分别为(2.87±0.26)、(1.91±0.11)、(0.24±0.10)μmolPi/mgprot.h,均明显低于对照组(3.28±0.35)μmolPi/mgpro.th(P<0.05);病理学观察显示,随着明矾的摄入,大鼠脑组织细胞出现间质水肿,细胞质疏松等病理变化。结论明矾可以影响大鼠脑组织细胞正常结构,并影响ATP酶活性及钙、镁元素的含量。
Objective To investigate the effect of food additive alum on ATPase activity, calcium and magnesium in rat brain. Methods Thirty-two male Wistar rats were randomly divided into four groups according to body weight: control group, low, middle and high dose alum group. After continuous feeding for 30 days, Na + K + -ATPase, Ca2 + -ATP Enzyme activity, Mg2 + -ATPase activity and the content of aluminum, calcium and magnesium in brain tissue, and the pathological observation of brain tissue. Results The contents of aluminum in brain tissue of low, medium and high dose alum group were (2.19 ± 0.39), (2.48 ± 0.33) and (3.52 ± 0.41) μg / g respectively, which were significantly higher than that of the control group (0.52 ± 0.12) μg / g (P <0.01). The contents of Mg and Mg2 + -ATPase in the brain tissue of high-dose group were significantly lower than those in the control group (P <0.05). The activities of Na + K + -ATPase in the low, medium and high dose groups were (2.87 ± 0.26), (1.91 ± 0.11) and (0.24 ± 0.10) μmol Pi / mgprot.h respectively, which were all significantly lower than that of the control group (3.28 ± 0.35) μmol Pi / mgpro.th With the intake of alum, rat brain tissue interstitial edema, cytoplasm and other pathological changes. Conclusion Alum can affect the normal structure of rat brain cells and affect ATPase activity and calcium and magnesium content.