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本文介绍了蟾蜍及新生大鼠离体背根神经节神经细胞膜电位及诱发动作电位的细胞内记录方法以及膜电导,翻转电位等电学特性的测量。应用上述方法测得蟾蜍DRG细胞膜输入电阻为30～40MΩ,新生大鼠的输入膜电阻为45～70MΩ;α_1肾上腺素能受体激活引起DRG细胞膜去极化反应时,膜电导减小.而α_2-肾上腺素能受体激活引起细胞膜电位超极化反应时,膜电导增加。去甲肾上腺素引起DRG细胞膜去极化反应的平均翻转电位值为-88.5mV。这些方法为神经细胞的某些电学特性、神经递质及受体等方面的研究提供了基本的实验手段。
In this paper, we report the intracellular recordings of membrane potential and evoked potential in isolated dorsal root ganglion neurons of Toad and neonatal rats and the measurement of electrical properties such as membrane conductance and turnover potential. Using the above method, the input resistance of DRG cell membrane was 30 ~ 40MΩ and the input membrane resistance of newborn rats was 45 ~ 70MΩ. The membrane conductance decreased when α_1 adrenergic receptor activated DRG membrane depolarization. - Adrenergic receptor activates the membrane potential hyperpolarization reaction, membrane conductivity increases. The average reversal potential value of norepinephrine-induced DRG cell membrane depolarization was -88.5 mV. These methods provide the basic experimental means for the study of some electrical properties of nerve cells, neurotransmitters and receptors.