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在综合防治血吸虫病的过程中,控制和消灭血吸虫的中间宿主——钉螺,实为一个很重要的环节,国内各地在控制和消灭钉螺的方法上有了很多丰富的经验,取得了一定的效果,但对钉螺的生物化学方面的研究,惜国内尚无很多文献报告,现将我们的工作报告如下。材料和方法钉螺来源于1962年4-5月采自上海青浦,江西上饶和玉山等地,室内是以滤纸泥缽饲养。试验方法:仿用陶义训等氏(1958)改良Seifter氏法。甲、试剂:(1)30％KOH溶液。(2)83％硫酸——硫酸(化学纯粹,比重1.84)100毫升与蒸馏水20毫升混和而成。(3)蒽酮试剂——0.2克蒽酮溶解于100毫升83％硫酸中,应用时当天配制。(4)标准葡萄糖溶液——将0.100克精制无水葡萄糖溶于100毫升蒸馏水中,作为储备
In the process of integrated prevention and control of schistosomiasis, the control and elimination of schistosomes intermediate host - snails, in fact, is a very important part of the domestic control and eliminate snails on the method has a lot of rich experience and achieved some results , But biochemical studies on snails, but there is not a lot of domestic literature report, we report the following work. Materials and Methods Snails from April 19 May 1962 from Shanghai Qingpu, Jiangxi Shangrao and Yushan and other places, the indoor filter paper mud is kept. Test Method: Imitation Tao Yi Xun’s (1958) improved Seifter’s method. A, Reagents: (1) 30% KOH solution. (2) 83% sulfuric acid - sulfuric acid (pure chemical, specific gravity 1.84) 100 ml and distilled water 20 ml mixed together. (3) Anthrone reagent - 0.2 g of anthrone is dissolved in 100 ml of 83% sulfuric acid and prepared on the day of application. (4) Standard glucose solution - Dissolve 0.100 g of refined anhydrous glucose in 100 ml of distilled water as a reserve