论文部分内容阅读
目的:建立雌性Wistar大鼠实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)模型,观察银杏叶提取物EGb761腹腔注射对EAE大鼠的可能疗效及神经保护作用和机制。方法:建立Wistar大鼠EAE模型,腹腔注射EGb761(70 mg/kg),连续7 d,于免疫后第14 d和第31 d取颈膨大,分别进行HE、LFB和改良BLS染色,观察各组大鼠炎细胞浸润、髓鞘脱失和轴突缺失情况。结果:(1)EGb761组与EAE组相比临床评分明显减低(P<0.05);(2)HE染色显示:EGb761组炎症浸润与EAE组相比明显减低(P<0.01);(3)LFB染色显示:EGb761脱髓鞘评分与EAE组相比明显降低(P<0.05);(4)改良Bielschowsky染色显示:EGb761组高峰期轴突平均缺失面积与EAE组相比明显下降(P<0.01)。结论:EGb761通过减轻炎细胞浸润、促进髓鞘再生和防止轴突缺失,从而对EAE大鼠临床症状的改善起到一定作用。
OBJECTIVE: To establish a model of experimental autoimmune encephalomyelitis (EAE) in female Wistar rats, and to observe the possible curative effect and neuroprotective effect and mechanism of intraperitoneal injection of Ginkgo biloba extract EGb761 on EAE rats. Methods: The Wistar rat model of EAE was established by intraperitoneal injection of EGb761 (70 mg / kg) for 7 days. The neck was swollen on the 14th and 31st day after immunization. HE, LFB and modified BLS staining were performed. Inflammatory cell infiltration, demyelination and axon loss in rats. Results: (1) The clinical score of EGb761 group was significantly lower than that of EAE group (P <0.05); (2) HE staining showed that the inflammatory infiltration in EGb761 group was significantly lower than that in EAE group (P <0.01); (3) Staining showed that the demyelination score of EGb761 was significantly lower than that of EAE group (P <0.05). (4) The modified Bielschowsky staining showed that the mean area of axon loss at peak of EGb761 group was significantly lower than that of EAE group (P <0.01) . Conclusion: EGb761 plays a role in the improvement of clinical symptoms in EAE rats by reducing inflammatory cell infiltration, promoting remyelination and preventing axonal degeneration.