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目的:探讨慢性胃粘膜损伤大鼠胃粘膜上皮细胞超微结构的改变和AST与AST-Ⅳ可能发挥的作用。方法:采用大黄灌胃造成大鼠慢性胃粘膜损伤模型,用扫描电镜观察慢性胃粘膜损伤大鼠胃粘膜表面上皮细胞超微结构的变化及AST与AST-Ⅳ预防给药和治疗给药对其的影响。结果:慢性胃粘膜损伤大鼠胃粘膜上皮细胞广泛坏死、破碎、形态模糊不完整,排列紊乱,细胞肿胀、扁平,细胞间无界限,多个细胞挤压扭结成团;细胞表面微绒毛稀少、脱落,分泌颗粒稀少;胃小凹结构畸形的。AST高(200mg/kg)、低(100mg/kg)剂量及AST-Ⅳ高剂量(4.08mg/kg),预防给药和治疗给药,均能够扭转慢性胃粘膜损伤大鼠胃粘膜上皮的病变,且AST各剂量的作用均优于AST-Ⅳ各剂量,AST高剂量预防给药和治疗给药的作用均明显优于其余各剂量,与正常组的水平相当;AST低剂量预防给药和治疗给药的作用均优于AST-Ⅳ高剂量。结论:慢性胃粘膜损伤大鼠的胃粘膜屏障受到破坏,AST及AST-Ⅳ均具有保护胃粘膜屏障的作用。AST的作用明显优于AST-Ⅳ,且随剂量的增加,AST的优越性更加明显,大剂量的作用接近正常组的水平。AST在较低剂量的情况下可以达到甚至超过AST-Ⅳ大剂量的作用效应。
Objective: To investigate the ultrastructural changes of epithelial cells in gastric mucosa of rats with chronic gastric mucosal injury and the possible roles of AST and AST-Ⅳ. Methods: Chronic gastric mucosa injury was induced by Rhubarb gavage. The ultrastructural changes of gastric mucosal epithelial cells in rats with chronic gastric mucosal injury were observed by scanning electron microscopy. AST and AST-Ⅳ were administered to patients with chronic gastric mucosal injury Impact. Results: The gastric mucosal epithelial cells of rats with chronic gastric mucosal injury were extensively necrotic and fragmented, the morphology was incomplete, disordered, swollen and flattened, with no boundaries between cells and many cells were squeezed and kinked into clusters. There were few microvilli on the cell surface, Exfoliation, secretion of rare particles; gastric concave concave structure. AST high dose (200mg / kg), low dose (100mg / kg) and high dose of AST-Ⅳ (4.08mg / kg) could prevent gastric mucosal epithelial lesions in rats with chronic gastric mucosal injury , And the effect of each dose of AST was better than that of AST-Ⅳ. The effect of high-dose AST prophylaxis and treatment was significantly better than that of the other groups, which was equivalent to that of normal group. Therapeutic drugs are superior to the high dose of AST-IV. Conclusion: Gastric mucosal barrier is damaged in rats with chronic gastric mucosal injury, and AST and AST-Ⅳ all play roles in protecting gastric mucosal barrier. The effect of AST was significantly better than that of AST-Ⅳ. With the increase of dose, the superiority of AST was more obvious, and the effect of high dose was close to that of normal group. AST can reach or surpass the high dose of AST-IV effect even at lower dosage.