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目的 :观察尼可地尔对大鼠脑缺血再灌注损伤的保护作用。方法 :采用Pulsinelli四动脉结扎法制作大鼠急性前脑缺血再灌注损伤的模型。大鼠随机分成 5组 ,每组 10只 ,剔除死亡和模型不成功后假手术组 6只、再灌注组 9只、尼莫地平 2mg·kg- 1组 6只、尼可地尔 5mg·kg- 1组 7只、尼可地尔10mg·kg- 1组 6只。于缺血前、再灌注前及再灌注过程中各iv给药 1次。结果 :缺血再灌注造成脑组织严重损伤 ,脑水份、脑钙及MDA含量显著增高 ,脑组织LDH含量明显减少 ,脑电图严重抑制 ,锥体细胞坏死明显。尼可地尔能有效地逆转上述变化(P <0 .0 5 )。结论 :尼可地尔具有明显的脑保护作用
Objective: To observe the protective effect of nicorandil on cerebral ischemia-reperfusion injury in rats. Methods: Acute forebrain ischemia-reperfusion injury was induced in rats by Pulsinelli four-artery ligation. The rats were randomly divided into 5 groups with 10 rats in each group. Six rats in the sham-operation group, 9 in the reperfusion group, 6 in the nimodipine 2 mg · kg-1 group and 5 mg · kg- - Group 1 7, Nicorandil 10 mg · kg-1 group 6. Before ischemia, reperfusion and reperfusion during the iv administration. Results: Cerebral ischemia-reperfusion caused serious damage of brain tissue, brain water content, brain calcium and MDA content was significantly increased, LDH content was significantly reduced in brain tissue, EEG was severely inhibited, pyramidal cell necrosis was obvious. Nicorandil effectively reversed these changes (P <0.05). Conclusion: Nicorandil has obvious protective effect on brain