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目的:探讨益气养阴消癥通络中药对早期糖尿病大鼠肾组织p38MAPK信号通路的作用。方法:将SD大鼠随机分为6组:单纯肾切组、模型组、厄贝沙坦组、中药低、中、高剂量组,按各组相应药物剂量灌胃。于第6周末检测各组大鼠24 h尿蛋白(UPro)量及肾功能水平;肾皮质p38MAPK mRNA,p-p38MAPK蛋白表达水平。结果:厄贝沙坦、中药低、中剂量组与模型组比较Upro量显著下降(P<0.05);治疗组与模型组比较,肾功能明显改善(P<0.05),且p38MAPK mRNA,p-p38MAPK蛋白表达水平显著下降(P<0.05)。结论:益气养阴消癥通络中药可能通过抑制p38MAPK信号通路表达,发挥对糖尿病肾病大鼠的治疗作用。
Objective: To investigate the effect of Yiqi Yangyin Xiaozhong Tongluo Chinese medicine on p38 MAPK pathway in renal tissue of early diabetic rats. Methods: The SD rats were randomly divided into 6 groups: simple nephrectomy group, model group, irbesartan group, low, medium and high dose Chinese medicine group, according to the corresponding drug dose in each group. At the end of the 6th week, the levels of urinary protein (UPro) and renal function of rats in each group were measured. The expressions of p38MAPK mRNA and p-p38MAPK protein in renal cortex were detected. Results: Compared with the model group, the levels of Upro in irbesartan and traditional Chinese medicine groups were significantly decreased (P <0.05), and the renal function was significantly improved in the treatment group compared with the model group (P <0.05) p38MAPK protein expression decreased significantly (P <0.05). Conclusion: Yiqi Yangyin Xiaozhong Tongluo Chinese medicine can inhibit the expression of p38MAPK signaling pathway and play a therapeutic role in diabetic nephropathy rats.