目的:研究P53及血管内皮生长因子(VEGF)在子宫颈癌及癌前病变组织中的表达及与宫颈癌发生和发展、生物学行为和预后的关系。方法:应用免疫组织化学和二步法检测P53及VEGF在50例宫颈慢性炎(CC)、30例宫颈尖锐湿疣(CA)、50例宫颈上皮内瘤变(CIN)和50例宫颈鳞癌(ICC)组织中的表达,并对其中50例宫颈癌患者进行随访分析。结果:1P53及VEGF-C在宫颈癌组织中的阳性表达率分别为74.00%(37/50)和82.00%(41/50),在宫颈上皮内瘤变组织中的阳性表达率分别为44.75%(22/50)和18.00%(9/50),在宫颈尖锐湿疣组织中的阳性表达率分别为13.33%(4/30)和10.00%(3/30),在宫颈慢性炎组织中的阳性表达率分别为2%(1/50)和0%(0/50),4组间3种指标的表达差异均有显著的统计学意义(P<0.05)。2宫颈癌组织中P53蛋白表达与淋巴结转移、病理分级相关(P<0.05),而与临床分期无关(P>0.05),P53蛋白高表达患者3年内生存率显著低于阴性组(P<0.05);VEGF蛋白表达与病理分级、淋巴结转移相关(P<0.01),而与临床分期无关(P>0.05)。结论:P53及VEGF蛋白作为肿瘤相关蛋白主要表达在宫颈癌及与癌发生相关的癌前病变CINⅢ级中,其表达与癌变过程呈递增关系,该蛋白表达促进癌演进,并与癌细胞转移、患者预后密切相关。P53及VEGFC的表达具有显著正相关性,具有协同促癌作用。VEGF和P53在CINⅢ及ICC中有明显的表达,是监测CIN及ICC的一个重要的指标。 Objective: To investigate the expression of P53 and vascular endothelial growth factor (VEGF) in cervical carcinoma and precancerous lesions and their correlation with the occurrence and development of cervical cancer, biological behavior and prognosis. Methods: The expressions of P53 and VEGF in 50 cases of chronic cervicitis (CC), 30 cases of cervical condyloma acuminatum (CA), 50 cases of cervical intraepithelial neoplasia (CIN) and 50 cases of squamous cell carcinoma of cervix were detected by immunohistochemistry and two- ICC) tissues, and 50 patients with cervical cancer were followed up. Results: The positive rates of 1P53 and VEGF-C in cervical cancer tissues were 74.00% (37/50) and 82.00% (41/50), respectively. The positive rates of 1P53 and VEGF-C in cervical intraepithelial neoplasia were 44.75% (22/50) and 18.00% (9/50) respectively. The positive rates in cervical condyloma acuminatum were 13.33% (4/30) and 10.00% (3/30) The expression rates of these three indexes were 2% (1/50) and 0% (0/50) respectively. There were significant differences among the three groups (P <0.05). P53 protein expression was correlated with lymph node metastasis and pathological grade in cervical cancer (P <0.05), but not with clinical stage (P> 0.05). The 3-year survival rate of P53 protein high expression group was significantly lower than that in negative group (P <0.05) ). VEGF protein expression was correlated with pathological grade and lymph node metastasis (P <0.01), but not with clinical stage (P> 0.05). CONCLUSION: P53 and VEGF proteins are mainly expressed in cervical cancer and precancerous lesions associated with carcinogenesis. The expression of P53 and VEGF is related to the progress of carcinogenesis. The expression of P53 and VEGF may promote the progression of cancer. The prognosis of patients is closely related. P53 and VEGFC expression has a significant positive correlation, with a synergistic role in promoting cancer. VEGF and P53 in CIN Ⅲ and ICC were significantly expressed, is an important indicator of monitoring CIN and ICC.