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目的 研究 Cp G寡核苷酸 (Cp G ODN)对日本血吸虫重组 2 8k Da GST(r Sj2 8GST)抗原分子的免疫佐剂作用和诱导 Th1型反应的效应。方法 用淋巴细胞增殖试验筛选对鼠有良好免疫刺激作用的 Cp G ODN;用基因工程方法生产 r Sj2 8GST抗原分子 ;EL ISA检测小鼠抗 r Sj2 8GST的同型抗体 Ig G1 、Ig G2 a水平。结果 筛选出的 Cp G ODN如 182 6、2 0 0 2、182 6 4、2 10 2等具有良好的免疫刺激活性 ,其 SI>2 ;纯化的 r Sj2 8GST抗原分子 ,呈单一电泳条带 ;以 Cp G ODN为 r Sj2 8GST免疫佐剂 ,其免疫小鼠产生的抗体滴度显著高于对照组 ,且同型抗体 Ig G2 a与 Ig G1 比值也明显升高。结论 Cp G ODN182 6 4是一种良好的免疫佐剂 ,并可显著诱导 Th1型免疫应答
Objective To investigate the effect of CpG ODN on immune adjuvant and induction of Th1 response in Schistosoma japonicum recombinant 2 8 kDa GST (r Sj2 8GST) antigen. Methods CpG ODN was screened by lymphocyte proliferation test. R Sj2 8GST antigenic gene was produced by genetic engineering method. The level of Ig G1 and Ig G2 a was detected by EL ISA in anti-r Sj2 8GST mouse. Results The CpG ODNs screened as 182 6,2 0 0 2,182 6 4,2 10 2 had good immunostimulatory activity with SI> 2. The purified rSj2 8GST antigen showed a single electrophoretic band. With CpG ODN as immunogen adjuvant rSj2 8GST, the antibody titers of mice immunized with CpG ODN were significantly higher than that of the control group, and the ratio of Ig G2 a to Ig G1 of the isotype antibody was also significantly increased. Conclusion CpG ODN182 6 4 is a good immune adjuvant, and can significantly induce Th1-type immune response