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为研究金银花不同采收期和不同部位提取液膜保护酶的活性与清除自由基的能力,采用生物化学分析法测定了不同采集期金银花花蕾、叶片和枝条膜保护酶活性,结果显示,SOD、POD和CAT活性从4月开始平稳上升,6月上旬前后达到最高值。叶片的上述指标最高,枝条最低。对自由基的清除能力也呈平稳上升趋势。金银花花蕾对超氧自由基和羟自由基的清除效应最高,花蕾和叶片对过氧化氢清除效应相当,枝条的最低。几种膜保护酶活性之间均呈较显著的正相关,当膜保护酶活性增强时,清除自由基的能力也随之提高,可能是它们的协调作用使金银花有较强的清除自由基的能力。
In order to study the activities of protective enzymes and the ability of scavenging free radicals in liquid membranes of honeysuckle collected at different harvesting periods and at different sites, biochemical assays were used to determine the protective enzyme activities of buds, leaves and shoots of honeysuckle at different collection stages. The results showed that The activity of SOD, POD and CAT increased steadily from April and peaked around the beginning of June. The above indicators of the leaves are the highest and the branches are the lowest. The ability to remove free radicals also showed a steady upward trend. The honeysuckle flower buds had the highest clearance effect on superoxide radicals and hydroxyl radicals, flower buds and leaves had equivalent hydrogen peroxide scavenging effect, and branches had the lowest. There was a significant positive correlation between the activities of several membrane-protecting enzymes. When the activity of membrane-protecting enzymes was increased, the ability to scavenge free radicals was also increased. It may be their coordinated effect that causes honeysuckle to have strong scavenging effects on free radicals. ability.