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目的通过检测胃癌Her-2/neu基因数量的变化,探讨Her/neu基因与胃癌预后的关系。方法应用双色荧光原位杂交技术(FISH),采用17号染色体着丝粒DNA探针和Her-2/neu基因探针,对21例胃癌进行检测。结果有18例胃癌获得满意的FISH信号,其中5例有Her-2/neu基因扩增(23.81%),有Her-2/neu基因扩增者淋巴结癌转移发生率是38.54%,无Her-2/neu基因扩增者淋巴结癌转移率是23.43%,P>0.05。肠型胃癌Her-2/neu基因扩增率是25%,弥漫性胃癌和混合型胃癌是9%及16%。结论 Her-2/neu基因扩增与胃癌患者的预后有明显关系,特别是有基因扩增的胃癌患者,术后死亡率高、生存期短。
Objective To explore the relationship between the Her / neu gene and the prognosis of gastric cancer by detecting the changes of Her-2 / neu gene number in gastric cancer. Methods Twenty-one cases of gastric cancer were detected by dual-color fluorescence in situ hybridization (FISH) using chromosome 17 centromere DNA probe and Her-2 / neu gene probe. Results FISH signals were obtained in 18 cases of gastric cancer, including 5 cases of Her-2 / neu gene amplification (23.81%), the incidence of lymph node metastasis with Her-2 / neu gene amplification was 38.54%, no Her- 2 / neu gene amplification lymph node metastasis rate was 23.43%, P> 0.05. The rate of Her-2 / neu gene amplification in intestinal-type gastric cancer was 25%, that of diffuse gastric and mixed gastric was 9% and 16%, respectively. Conclusions The amplification of Her-2 / neu gene has a significant relationship with the prognosis of patients with gastric cancer. Especially in patients with gastric cancer with gene amplification, the postoperative mortality is high and the survival time is short.